Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Resin bead micro-UV-visible absorption spectroscopy.

Lu Shin Wong1, Fabrice Birembaut, William S Brocklesby

  • 1School of Chemistry, University of Edinburgh, Edinburgh EH9 3JJ, UK.

Analytical Chemistry
|April 2, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Caspofungin-Based Red-Emissive Probes for Fluorescent Imaging of Pathogenic Fungi.

JACS Au·2026
Same author

Product distribution in the silicatein-catalysed synthesis of polydimethylsiloxane.

Catalysis science & technology·2026
Same author

The future of fundamental science led by generative closed-loop artificial intelligence.

Frontiers in artificial intelligence·2026
Same author

Structure-Property Relationships of Near-Infrared Cyanine Dyes: Chalcogen-Driven Singlet Oxygen Generation with High Fluorescence Efficiency.

ACS omega·2026
Same author

Author Correction: A fluorogenic, peptide-based probe for the detection of Cathepsin D in macrophages.

Communications chemistry·2026
Same author

Enhanced stability and reusability of recombinant silicatein upon biomimetic metal-organic framework crystallization.

Chemical science·2025
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
See all related articles

A novel microscope-spectrometer system analyzes solid-supported dyes, correlating spectral data with solution measurements. This enables dye-loaded beads to function as reliable pH sensors.

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Materials Science

Background:

  • Dyes are crucial for various applications, but their behavior on solid supports requires precise characterization.
  • UV-Vis spectroscopy is a standard technique for analyzing dye properties.

Purpose of the Study:

  • To develop and validate a microscope-spectrometer system for analyzing solid-supported dyes.
  • To assess the feasibility of using dye-loaded solid supports as pH sensors.

Main Methods:

  • Construction of a microscope coupled with a miniature UV-Vis spectrometer.
  • Analysis of dyes immobilized on solid supports and comparison with dyes in solution.
  • Measurement of spectral profiles at various pH levels to determine pK(a) values.

Related Experiment Videos

Main Results:

  • The system showed good correlation in spectral shape and lambda(max) values between solid-supported and solution-based dyes.
  • A linear relationship was observed between dye loading and UV-Vis absorbance.
  • Accurate pK(a) values were determined for dyes on beads, comparable to solution values.

Conclusions:

  • The developed microscope-spectrometer is effective for characterizing solid-supported dyes.
  • Dye-loaded beads can serve as effective and quantifiable pH sensors.
  • This technology offers a new approach for developing solid-state chemical sensors.