Related Experiment Videos
Antibody capture from corn endosperm extracts by packed bed and expanded bed adsorption
Todd J Menkhaus1, Charles E Glatz
1Department of Chemical Engineering, 2114 Sweeney Hall, Iowa State University, Ames, Iowa 50011-2230, USA.
Biotechnology Progress
|April 2, 2005
Summary
Plant-based antibody production offers a scalable solution for chronic infection treatments. This study compares packed bed and expanded bed adsorption for purifying antibodies from corn, finding packed beds more cost-effective.
Area of Science:
- Biotechnology
- Protein Purification
- Plant Molecular Farming
Background:
- Topical monoclonal antibody treatments for chronic infections necessitate large-scale antibody production.
- Plants are promising hosts for producing multisubunit antibodies due to their scalability.
- Efficient and cost-effective purification methods are crucial for enabling plant-based antibody applications.
Purpose of the Study:
- To evaluate and compare packed bed and expanded bed adsorption (EBA) techniques for purifying human secretory antibodies from corn endosperm extracts.
- To assess the impact of different adsorbent properties and process parameters on purification yield and efficiency.
- To determine the most economically viable purification strategy for plant-produced antibodies.
Main Methods:
- Modeled antibody purification using extracts from nontransgenic corn endosperm.
- Compared packed bed adsorption with SP-Sepharose FF resin against various EBA adsorbents.
- Evaluated adsorbent performance based on yield, purification factor, and operational costs.
- Analyzed flow distribution using residence time distribution analysis for different inlet designs.
Main Results:
- Complete clarification followed by packed bed adsorption yielded 75% antibody product with 2.3-fold purification.
- EBA with UpFront SP-steel core resin achieved 61% yield and 2.1-fold purification, but with higher operating costs.
- Corn endosperm proved advantageous due to significantly lower contaminating proteins compared to other plant sources.
- All tested inlet designs provided adequate flow distribution for EBA.
Conclusions:
- Packed bed adsorption offers a more cost-effective and efficient method for purifying antibodies from corn endosperm compared to EBA.
- The choice of resin and process design significantly impacts purification performance and economics.
- Corn endosperm is a suitable starting material for large-scale antibody production, facilitating lower recovery costs.