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Related Experiment Videos

Spherical alginate granules formulated for quick-release active subtilisin.

Zhaohui M Liu1, Todd Becker, Ronald J Neufeld

  • 1Department of Chemical Engineering, Queen's University, Kingston, Ontario, Canada K7L 3N6.

Biotechnology Progress
|April 2, 2005
PubMed
Summary

Novel enzyme granules were created using alginate and internal gelation, offering a robust and dispersible alternative for industrial applications like detergents. This new granulation method enhances enzyme stability and performance.

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Area of Science:

  • Biotechnology
  • Materials Science
  • Chemical Engineering

Background:

  • Industrial enzyme applications require robust and active enzyme formulations.
  • Established granulation methods like fluid bed coating have limitations.
  • Developing novel, efficient granulation techniques is crucial for enzyme delivery.

Purpose of the Study:

  • To develop a new method for creating attrition-resistant, spherical enzyme granules.
  • To encapsulate active subtilisin within alginate microspheres using internal gelation.
  • To optimize formulation and processing for enhanced granule properties and enzyme activity.

Main Methods:

  • Emulsification of alginate sol with active subtilisin.
  • Internal gelation via in situ calcium ion release.

Related Experiment Videos

  • Particle separation and acetone extractive drying.
  • Characterization of granule size, morphology, mechanical strength, and solubility.

    • Main Results:

    • Successfully produced spherical, attrition-resistant enzyme granules with mean diameter of 500 microm.
    • Granules exhibited high activity, dispersibility, and mechanical robustness.
    • Optimized formulations (e.g., 3% SG150 alginate, 10% starch) yielded high compression resistance and low dust.
    • Subtilisin mass yields were ~50% with specific activity yields of 60-100%.

    Conclusions:

    • Internal gelation of alginate offers a novel and attractive alternative for enzyme granulation.
    • The developed technique produces robust, active, and dispersible enzyme granules suitable for industrial use.
    • Formulation parameters significantly influence granule properties, enabling tailored applications in detergents, textiles, and food processing.