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Updated: Aug 18, 2026
Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
Published on: July 16, 2019
Interferon-gamma associated cytokines and chemokines produced by spleen cells from Brucella-immune mice
Chrysanthi Paranavitana1, Elzbieta Zelazowska, Mina Izadjoo
1Department of Bacterial Diseases, Walter Reed Army Institute of Research, Bldg 503, Room 3E10, Robert Grant Avenue, Silver Spring, MD 20910, USA. chrysanthi.paranavitana@na.amedd.army.mil
Abstract:
It is known that interferon (IFN)-gamma plays a critical role in protection against brucellosis. In this study we have investigated several cytokines and chemokines that are associated with IFN-gamma for potential in vitro correlates of protection. We cultured spleen cells in vitro from mice immunized orally with a live, attenuated Brucella melitensis vaccine candidate (WR201) and stimulated these cells with a lysate of B. melitensis. Differential gene expression of several cytokines and chemokines in stimulated spleen cells was analysed by real-time PCR, and secreted proteins were determined by ELISA. Immunized mice produced higher levels of both protein and gene transcripts for IFN-gamma, interleukin (IL)-2, IL-18 and MIP1-alpha. Immunized mice also had elevated gene expression levels for IL12-p40, IL23-p19, IP-10, MIG and MCP-1 when compared to normal mice. In this study we have identified new cytokines and chemokines as potential immune correlates in responses to protection in Brucella-vaccinated mice.
