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Updated: Aug 18, 2026

Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
Antinuclear antibodies
1Division of Connective Tissue Disease and Autoimmunity, Department of Dermatology Nagoya University Graduate School of Medicine 65 Tsurumai-cho, Showa-ku Nagoya 466-8550 Japan. ymuro@med.nagoya-u.ac.jp
Abstract:
The presence of abnormal levels of autoantibodies to intracellular antigens is a hallmark of systemic connective tissue disease. The indirect immunofluorescence assay is the most commonly used routine test for the detection of antinuclear antibodies. In this text, several representative patterns of fluorescence are reviewed and some pitfalls for application of the results to the clinical field are mentioned.
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