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Related Concept Videos

Imaging and Quantification of Axonal Regeneration in Axotomized Rat Retinal Ganglion Neurons02:23

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Source: Portela-Lomba, M., et. al. Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration. J. Vis. Exp. (2020).This video demonstrates imaging and quantification of axonal regeneration in axotomized rat retinal ganglion neurons (RGNs) co-cultured with immortalized human olfactory ensheathing glia cells. RGNs are immunolabeled, with red somas and green axons. Images were captured using an epifluorescence microscope,...
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The pericytes in retinal vasculature were examined by immunofluorescent staining with platelet-derived growth factor receptor β after retro-orbital injection of fluorescent tomato lectin. The labeled retina was further treated with the tissue-clearing method and whole mounted for visualizing the three-dimensional views of pericytes surrounding retinal vasculature under a confocal...
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This article describes a method for measuring retinal vasculature reactivity in vivo with human subjects using a gas breathing provocation technique to deliver vasoactive stimuli while acquiring retinal...
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Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial...
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The rodent retina has long been recognized as an accessible window to the brain. In this technical paper we provide a protocol that employs the mouse model of oxygen-induced retinopathy to study the mechanisms that lead to failure of vascular regeneration within the central nervous system after ischemic injury. The described system can also be harnessed to explore strategies to promote regrowth of functional blood vessels within the retina and...
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This video demonstrates a procedure to induce retinal degeneration in adult zebrafish using laser injury. The laser targets photoreceptor cells in the retina, triggering cell death and a degenerative process. This damage activates Müller glia (MG) cells, leading to their dedifferentiation into stem cell progenitors that aid in retinal...
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Related Experiment Video

Updated: Jan 20, 2026

Retinal Vascular Reactivity as Assessed by Optical Coherence Tomography Angiography
07:23

Retinal Vascular Reactivity as Assessed by Optical Coherence Tomography Angiography

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Retinal vascular regeneration.

Atsushi Otani1, Martin Friedlander

  • 1Kyoto University, Japan. otan@kuhp.kyoto-u.ac.jp

Seminars in Ophthalmology
|April 5, 2005
PubMed
Summary
This summary is machine-generated.

Stem cells, specifically endothelial progenitor cells (EPCs), show promise for treating retinal diseases through therapeutic angiogenesis. Beyond ischemic conditions, EPC therapy may benefit degenerative disorders and enable cell-based gene therapy, though safety requires evaluation.

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Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Cell Therapy

Background:

  • Retinal diseases pose significant challenges to vision.
  • Therapeutic angiogenesis aims to restore blood vessel formation.
  • Stem cells offer potential for novel treatment strategies.

Purpose of the Study:

  • To explore the use of stem cells, particularly endothelial progenitor cells (EPCs), for therapeutic angiogenesis in retinal diseases.
  • To evaluate the potential applications of EPCs beyond ischemic conditions, including degenerative disorders and gene therapy.
  • To highlight the advantages and considerations of autologous bone marrow-derived EPC therapy.

Main Methods:

  • Discussion of existing research and potential applications.
  • Focus on endothelial progenitor cells (EPCs) derived from bone marrow.
  • Consideration of autologous grafting for patient-specific treatment.

Main Results:

  • EPCs demonstrate potential utility in treating ischemic retinal diseases.
  • EPCs may also be applicable to retinal degenerative disorders.
  • EPCs could be utilized for cell-based gene therapy approaches.

Conclusions:

  • Stem cell therapy, specifically using EPCs, holds significant therapeutic potential for a range of retinal conditions.
  • Autologous bone marrow-derived EPCs offer advantages, including the possibility of self-grafting.
  • Further evaluation of potential toxicities and cell growth control is crucial before clinical implementation.