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Updated: Aug 18, 2026

Anti-Nuclear Antibody Screening Using HEp-2 Cells
13:01

Anti-Nuclear Antibody Screening Using HEp-2 Cells

Published on: June 23, 2014

Detection of antibodies to the extractable nuclear antigens by enzyme linked immunosorbent assay

Khalil A Aziz1, Abdul A Faizal

  • 1Regional Department of Immunology, Birmingham Heartlands Hospital, Bordesley Green East, Birmingham B9 5SS', United Kingdom. khalilazizh@yahoo.co.uk

Saudi Medical Journal
|April 5, 2005
PubMed
Abstract

No abstract available in PubMed .

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.