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Interaction between proliferating cell nuclear antigen (PCNA) and a DnaJ induced by DNA damage
Taichi Yamamoto1, Yoko Mori, Toyotaka Ishibashi
1Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
Journal of Plant Research
|April 5, 2005
Summary
Rice proliferating cell nuclear antigen (OsPCNA) interacts with rice DnaJ protein (OsDnaJ). OsDnaJ expression is induced by DNA damage, suggesting a role in DNA repair alongside OsPCNA.
Area of Science:
- Molecular Biology
- Plant Science
- Genetics
Background:
- Proliferating cell nuclear antigen (PCNA) is crucial for DNA replication and repair.
- Understanding plant PCNA interactions is key to comprehending DNA maintenance mechanisms.
Purpose of the Study:
- To identify and characterize proteins interacting with rice PCNA (OsPCNA).
- To investigate the function and expression patterns of a novel interacting protein, OsDnaJ.
Main Methods:
- Two-hybrid analysis was used to identify interacting partners of OsPCNA.
- In vitro binding assays confirmed the interaction between OsPCNA and OsDnaJ.
- Gene expression analysis was performed using quantitative PCR and by observing mRNA induction.
Main Results:
- OsPCNA was found to interact with a rice DnaJ protein, designated OsDnaJ.
- OsDnaJ demonstrated binding to OsPCNA in vitro.
- OsDnaJ transcripts were highly expressed in proliferating cells and induced by UV and H2O2.
- OsPCNA and OsDnaJ exhibited similar expression patterns.
Conclusions:
- OsDnaJ is a novel interacting partner of OsPCNA in rice.
- OsDnaJ's expression patterns suggest a role in DNA repair pathways, potentially in conjunction with OsPCNA.
- Further research is warranted to elucidate the precise functional relationship between OsPCNA and OsDnaJ in DNA replication and repair.