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[Determination of prostaglandinum E1 by fluorescence extinction method]
Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|August 1, 1997
Summary
This study shows methyl alcohol fluorescence is quenched by Prostaglandin E1 (PG E1). A linear relationship was found between fluorescence extinction and PG E1 concentration, confirming its utility.
Area of Science:
- Analytical Chemistry
- Biochemistry
Context:
- Prostaglandin E1 (PG E1) is a biologically significant lipid compound.
- Fluorescence quenching is a sensitive analytical technique.
Purpose:
- To investigate the fluorescence extinction effect of methyl alcohol induced by Prostaglandin E1 (PG E1).
- To establish a quantitative relationship between fluorescence extinction intensity and PG E1 concentration.
Summary:
- A linear correlation was observed between fluorescence extinction intensity and PG E1 concentration in methyl alcohol.
- The linear equation is deltaF = 0.222c + 9.33 x 10(-2), with a correlation coefficient r = 0.99930.
- Results from the fluorescence extinction method align with those from the violet spectrophotometric method.
Impact:
- This fluorescence quenching method provides a sensitive and accurate approach for quantifying PG E1.
- The findings support the application of fluorescence spectroscopy in biochemical analysis.