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[The construction of recombinant plasmid with prostate-specific membrane antigen promoter controlling reporter gene
Hao Zeng1, Hong Li, Yong-chuan Liao
1Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To study the specificity of prostate-specific membrane antigen (PSMA) promoter in controlling gene expression.
Methods:
PSMA promoter gene was amplified with PCR, and then the promoter gene was cloned into the vector pEGFP-1 to construct a recombinant plasmid, which was transfected into different cell lines such as LNcap, PC-3, MCF-7, A549. Green fluorescent protein (GFP) expression was observed.
Results:
The recombinant plasmid constructed, and PSMA promoter uniquely showed modulating activity in PSMA positive cell line.
Conclusion:
PSMA promoter possesses PSMA positive cell specificity, as well as prostatic tissue specificity. PSMA promoter may have the potential for use in targeted gene therapy of prostate adenocarcinoma.
