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Updated: Aug 18, 2026
Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
Published on: July 14, 2017
[The effect of ultraviolet B on interleukin-8 secretion in human keratinocyte cell line]
Xian Jiang1, Guo-xing Wang, Min-yan Zhou
1Department of Dermatology, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To investigate the effect of ultraviolet B (UVB) on Interleukin-8 secretion in human keratinocyte cell line.
Methods:
The concentration of IL-8 was detected by ELISA kit 24 h after human keratinocytes (HKC) were irradiated by different doses of UVB, and the level of IL-8 was also determined at different times after the same dose of UVB irradiation.
Results:
The secretion of IL-8 was increased after the HKC were irradiated by UVB, the effect was dose-dependent when UVB ranged from 10 to 40 mJ/cm2, and there was statistically significant difference between the IL-8 level of UVB groups (20-70 mJ/cm2) and the control (0 mJ/cm2) (P<0.01). The level of IL-8 was increased 1 h after the irradiation of 30 mJ/cm2 UVB, and it reached the peak at 12 h. There was statistically significant difference between the IL-8 levels detected at different times (3 h, 6 h, 12 h, 24 h after irradiation) and that at 0 h (P<0.01).
Conclusion:
UVB increase the secretion of IL-8, and the effect is dose-dependent to some extent.
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