[Purification of human serum apolipoprotein A I and A II by middle-pressure liquid chromatography]

This study is made for the purification of human HDL apolipoproteins by middle-pressure liquid chromatography (MPLC).

Human HDL was isolated by one step density ultracentrifugation. Delipided human HDL was separated by Sephacryl S-200 molecular sieve chromatography. Sephadex G-75 molecular sieve chromatography was used to separate apoA I and apoA II.

Purified apoA I and apoA II were obtained and SDS-PAGE and immunodiffusion test indicated that the proteins are the same as those theoretically predicted.

We have established a purification procedure for human HDL apolipoproteins with high efficiency and simplicity by MPLC. It could serve as a base for preclinical and clinical trials of HDL apolipoproteins.