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Ca2+ translocation in Ehrlich ascites tumor cells.

R Hinnen, H Miyamoto, E Racker

    The Journal of Membrane Biology
    |September 14, 1979
    PubMed
    Summary
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    The Journal of experimental medicine·2009

    Calcium (Ca2+) uptake in tumor cells was investigated at low temperatures. Researchers identified a potential Ca2+/H+ antiport mechanism and an energy-dependent Ca2+ expulsion system involving a Ca2+-dependent ATPase.

    Area of Science:

    • Cellular Physiology
    • Biochemistry
    • Membrane Transport

    Background:

    • Understanding calcium (Ca2+) transport in tumor cells is crucial for comprehending cellular function and developing therapeutic strategies.
    • Previous studies implicated various ions and conditions in Ca2+ handling, but a clear mechanism in Ehrlich ascites tumor cells remained elusive.

    Purpose of the Study:

    • To elucidate the mechanism of Ca2+ uptake in Ehrlich ascites tumor cells under controlled conditions.
    • To identify potential transporters and energy-dependent systems involved in Ca2+ homeostasis.

    Main Methods:

    • Studied Ca2+ uptake at 0°C with mitochondrial inhibitors to prevent organelle sequestration and excretion.
    • Utilized specific inhibitors like Ruthenium Red, Valinomycin, and Nigericin to probe transport mechanisms.

    Related Experiment Videos

  • Analyzed plasma membrane preparations and reconstituted proteoliposomes to identify Ca2+-dependent ATPase activity.

    • Main Results:

    • Ruthenium Red inhibited Ca2+ uptake, while Pi and Mg2+ had no effect.
    • Valinomycin and Nigericin modulated Ca2+ transport, suggesting involvement of ion gradients and electrogenic transport.
    • Glucose inhibition indicated an energy-dependent Ca2+ expulsion mechanism, supported by the identification of a Ca2+-dependent ATPase in plasma membrane preparations.

    Conclusions:

    • Proposed an electrogenic Ca2+ uptake mechanism, potentially a Ca2+/H+ antiport, in Ehrlich ascites tumor cells.
    • Confirmed the presence of an energy-dependent Ca2+ expulsion system linked to a plasma membrane Ca2+-dependent ATPase.
    • These findings provide insights into Ca2+ regulation in tumor cells and potential targets for intervention.