Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Axonal isoforms of myosin-I.

Linda M Lund1, Victor M Machado, Irvine G McQuarrie

  • 1Louis Stokes Cleveland VA Medical Center, Case Western Reserve University, USA. llund001@umaryland.edu

Biochemical and Biophysical Research Communications
|April 6, 2005
PubMed
Summary

Myosin-Igamma (myr4) is abundant in spinal motor neurons and axon regrowth. This mechanoenzyme is transported via slow axonal transport to axonal membranes, aiding in nerve repair.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromodomain helicase binding protein 8 (Chd8) is a novel A-kinase anchoring protein expressed during rat cardiac development.

PloS one·2012
Same author

Synemin isoforms differentially organize cell junctions and desmin filaments in neonatal cardiomyocytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2011
Same author

Perilipin 5, a lipid droplet-associated protein, provides physical and metabolic linkage to mitochondria.

Journal of lipid research·2011
Same author

Intra-axonal myosin and actin in nerve regeneration.

Neurosurgery·2009
Same author

The intermediate filament protein, synemin, is an AKAP in the heart.

Archives of biochemistry and biophysics·2006
Same author

Correlates for posttraumatic stress disorder in Gulf War veterans: a retrospective study of main and moderating effects.

Journal of anxiety disorders·2005

Area of Science:

  • Neuroscience
  • Cell Biology

Background:

  • Myosin-Igamma (myr4) is a mechanoenzyme present in high concentrations during neonatal axon tract formation.
  • Understanding the role of myr4 in neuronal function and repair is crucial.

Purpose of the Study:

  • To characterize the mechanoenzyme myosin-Igamma (myr4) in Sprague-Dawley rat spinal motor neurons.
  • To investigate the localization and transport of myr4 within axons.

Main Methods:

  • Raising an antibody against myr4.
  • In situ hybridization using riboprobes to detect myr4 mRNA.
  • Immunolabeling of nerves undergoing Wallerian degeneration.

Main Results:

  • Myr4 mRNA was abundant in spinal cord motor neurons, especially during axon regrowth.
  • Anti-myr4 labeled the axolemma and SER in degenerating nerves after cytoskeletal components degraded.
  • Newly synthesized myr4 was transported via the slow component (SC) of axonal transport (1-8 mm/day), but not the fast component (FC).

Conclusions:

  • The slow component of axonal transport delivers myr4 to the cytoplasmic surfaces of axonal membranes (SER and axolemma).
  • This localization anchors the tail domain of myr4, allowing the head domain to interact with F-actin.
  • Myr4 plays a role in axonal membrane dynamics and potentially nerve repair processes.

Related Experiment Videos