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Updated: Aug 18, 2026

Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation
Published on: November 4, 2021
[The study on the treatment of Oviducts Ranae protein with enzyme hydrolysis]
1Harbin Business University, Harbin 150040, China. sunximi@yeah.net
Abstract:
The paper introduced a process to enhance the yield of Oviducts Ranae protein using in vitro enzyme hydrolysis. The treatment process included two steps: (1) a 3 - 4 h of hydrolysis of a 0.025 g x g(-1) concentration of substance, at pH 7 and 60 degrees C, using 4% of papain; and (2) followed with a 6 - 8 h hydrolysis, at pH 2 - 2.5 and 60 degrees C, using 3% of pepsin. This treatment process significantly improved the lyophilized Oviducts Ranae in solubility and fluidity, which is convenient for the relative pharmaceutical preparations.
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