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Characterization of cells with osteogenic potential from human marrow
S E Haynesworth1, J Goshima, V M Goldberg
1Department of Biology, Case Western Reserve University, Cleveland, OH 44106.
Bone
|January 1, 1992
Summary
Human bone marrow contains mesenchymal stem cells capable of forming bone. These cells, when cultured and implanted in porous ceramics, demonstrated significant osteogenic potential in vivo.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Regenerative Medicine
Background:
- Previous studies indicated animal bone marrow cells could differentiate into bone and cartilage.
- The potential for human bone marrow cells in these processes remained less understood.
Purpose of the Study:
- To investigate the osteogenic and chondrogenic potential of human bone marrow-derived cells.
- To compare the efficacy of two in vivo assays for evaluating this potential.
Main Methods:
- Human bone marrow cells were cultured and expanded.
- Cells were implanted into porous calcium phosphate ceramics or diffusion chambers.
- Subcutaneous and peritoneal implantation in nude mice were utilized for in vivo assays.
- Histological and immunocytochemical analyses were performed.
Main Results:
- Bone formation was observed in ceramic implants containing human marrow-derived cells.
- Immunocytochemistry confirmed the bone originated from human cells.
- No cartilage formation was detected in any assay.
- Diffusion chamber assays did not yield bone or cartilage formation.
Conclusions:
- Human bone marrow contains cells with osteogenic potential, which can be enriched and expanded in culture.
- Subcutaneous implantation in porous calcium phosphate ceramics is a sensitive in vivo assay for assessing osteogenic potential.
- Diffusion chambers appear less sensitive for evaluating osteogenic lineage potential.