Related Experiment Video
Updated: Jul 17, 2026

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
Published on: February 25, 2016
Nitrosylation of thiols in vascular homeostasis and disease
Antonio Martínez-Ruiz1, Santiago Lamas
1Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Sinesio Delgado, 4, E-28029, Madrid, Spain. amartinez@cnic.es
Abstract:
Post-translational modification of thiols to form a nitrosothiol (S-nitrosylation) is gaining attention as a mechanism by which nitric oxide can exert some of its effects in the cardiovascular system, as well as in other systems. It has been proposed that this modification would have a particularly important role in cell signaling. However, its study has been hampered by many technical difficulties. In this paper, we summarize current achievements in the field that may help to answer the questions that have been posed about the functional role of this modification. Some of these achievements have arisen from methodologic improvements, such as proteomic studies or approaches to identify subcellular localization and tissue distribution of the modification. New functional consequences of the modification of individual proteins are also summarized.
More Related Videos
07:16Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
08:23Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Related Concept Videos
Preparation and Reactions of Thiols
Nitric Oxide Signaling Pathway
Transducer Mechanism: Enzyme-Linked Receptors
Major types that are helpful drug targets include:
Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
Coronary Artery Disease II: Pathophysiology
Sulfur Assimilation