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Direct interaction and cooperative role of tumor suppressor p16 with band 3 (AE1)
Guo-Hui Fu1, Yong Wang, Yu-Hui Xi
1Department of Pathophysiology, Shanghai Institute of Hematology, Rui-Jin Hospital, Shanghai Second Medical University (SSMU), Shanghai 200025, PR China. guohuifu@shsmu.edu.cn
Abstract:
By using the C-terminal 112-residue of band 3 to screen the K562 cDNA library, we find that the p16 interacts with band 3, which was confirmed both in yeast and in mammalian cells. Functional experiments show that p16 facilitates the movement of band 3 to plasma membrane with increased anion transport activity in 293t cells. Moreover, expression of endogenous p16 in 293t cells was increased at 24 and 36 h after transfection with band 3. Our findings provide a novel regulation pathway for both band 3 and p16.
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