Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Asymmetric binding between SecA and SecB two symmetric proteins: implications for function in export.

Linda L Randall1, Jennine M Crane, Angela A Lilly

  • 1Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA. craneje@missouri.edu

Journal of Molecular Biology
|April 7, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biomass carbonized P-V-W/C-dots/g-C₃N₄ with acid-metal synergistic catalysis and anchoring regulatory mechanism for deep oxidative desulfurization.

Journal of colloid and interface science·2026
Same author

Preclinical and clinical evaluation of LY3451838, a PACAP-neutralizing monoclonal antibody, in randomized, double-blind, placebo-controlled phase 1 and phase 2 studies involving healthy adults and adults with treatment-resistant migraine.

Cephalalgia : an international journal of headache·2025
Same author

Prominent Oxidative Desulfurization with an Advanced Colloids Catalyst under Mild Conditions: Synergistic Interaction of Interface-Phase Transfer.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Characterization of human XPD helicase activity with single-molecule magnetic tweezers.

Biophysical journal·2023
Same author

Microstructure optimization strategy of ZnIn<sub>2</sub>S<sub>4</sub>/rGO composites toward enhanced and tunable electromagnetic wave absorption properties.

Dalton transactions (Cambridge, England : 2003)·2023
Same author

Binding, Neutralization and Internalization of the Interleukin-13 Antibody, Lebrikizumab.

Dermatology and therapy·2023

SecB chaperone protein interacts asymmetrically with SecA, facilitating precursor polypeptide export in E. coli. This binding mechanism is key for protein translocation across cellular membranes.

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Protein Transport

Background:

  • SecB is a tetrameric chaperone in E. coli crucial for protein export.
  • It maintains precursor polypeptides in a non-native state and specifically binds to SecA.

Purpose of the Study:

  • To investigate the molecular interactions between SecB and SecA during protein export.
  • To elucidate the mechanism by which SecB facilitates precursor transfer to SecA.

Main Methods:

  • Utilized variants of SecA and SecB proteins.
  • Analyzed the binding interfaces and interactions between these variants.

Main Results:

  • Demonstrated an unexpected asymmetric binding between the symmetric SecB and SecA molecules.

Related Experiment Videos

  • Identified that C-terminal alpha-helices of SecB bind to the SecA dimer interface.

    • Conclusions:

    • SecB binding disrupts the SecA dimer interface, inducing conformational changes.
    • This disruption is essential for efficient precursor transfer from SecB to SecA for translocation.