Related Experiment Video
Updated: Aug 18, 2026

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
Published on: May 19, 2012
Ethyl alpha-D-glucoside was absorbed in small intestine and excreted in urine as intact form
Tomoyuki Mishima1, Takashi Hayakawa, Kenji Ozeki
1The United Graduate School of Agricultural Science, Gifu University, Gifu, Japan. h6103007@guedu.cc.gifu-u.ac.jp
Objective:
Ethyl alpha-D-glucoside (alpha-EG) is a peculiar component in sake. We investigated how alpha-EG was absorbed, hydrolyzed, and excreted in urine when it was ingested orally by rats.
Methods:
Hydrolyzing activity for alpha-EG was determined by incubating it with crude enzyme solutions prepared from several rat organs, and absorption activity for alpha-EG was determined by incubating rat small intestinal everted sac in sodium or potassium Krebs-Ringer buffer that contained alpha-EG. alpha-EG solution was fed to rats, and urine volume and plasma alpha-EG, glucose and insulin and urinary alpha-EG were determined.
Results:
alpha-EG was hydrolyzed by crude enzyme solutions prepared from rat small intestinal mucosa and kidney, and these hydrolyzing activities were lower than those for maltose. alpha-EG absorbed into everted rat intestinal sacs in potassium Krebs-Ringer buffer reduced almost completely compared with that in sodium Krebs-Ringer buffer. When alpha-EG was ingested orally by rats, it was absorbed into the bloodstream and more than 60% was excreted in urine, and urine volume increased.
Conclusions:
In rats, alpha-EG was absorbed in small intestine and excreted intact in urine without affecting blood glucose and insulin and thus was a diuretic, insulin-independent, and low-nutritive glucoside that could be safely applicable to food.
More Related Videos
Related Concept Videos
Oral Hypoglycemic Agents: α-Glucosidase Inhibitors
Acarbose and miglitol are typically...
Drug Elimination by Renal Route: Tubular Reabsorption
Glucose Absorption Into the Small Intestine
Drug Excretion: Miscellaneous Routes
Drug Absorption: Factors Affecting GI Absorption
In...
Phase II Reactions: Glucuronidation

