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Co-immunoprecipitation of the Mouse Mx1 Protein with the Influenza A Virus Nucleoprotein
Published on: April 21, 2015
Resistance of paramyxoviridae to type I interferon-induced Bos taurus Mx1 dynamin
Michael Leroy1, Etienne Baise, Grégory Pire
1Department of Pathology, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.
Abstract:
Typical targets of type I interferon (IFN)-induced antiviral Mx proteins known to date have been shown to share a common profile: single-stranded negative-sense RNA viruses. Among them, human MxA is known to interfere with the replication of measles, human, and bovine parainfluenza-3 viruses (BoPi3V), that is, three members of the Paramyxoviridae family. Recently, bovine Mx1 protein (BoMx1) was included in the group of Mx proteins with authenticated antiviral potential, as it dramatically represses the replication of vesicular stomatitis virus (VSV). As replication in bovine cells of Pi3, respiratory syncytial (RS), and Sendai (Se) viruses, all members of the same family, is known to be reduced on IFN-alpha incorporation into the culture medium, it was hypothesized that the BoMx1 pathway possibly was involved, its antiviral spectrum thus probably extending to Paramyxoviridae. In this study, probing of BoMx1-inhibiting effects was carried out by infecting a transgenic Vero cell line that allows tightly regulated conditional expression of BoMx1 after doxycycline treatment with a wide array of Paramyxoviridae. Expressing and nonexpressing cells displayed similar viability, cytopathic effects (CPEs), and amounts of infectious virus yields, whatever the infecting virus or the multiplicity of infection (moi) imposed. It is, therefore, concluded that BoMx1 does not interfere with Paramyxoviridae.
Insights
Bovine Mx1 protein (BoMx1) was investigated for its antiviral activity against Paramyxoviridae viruses. This study found that BoMx1 does not interfere with the replication of these viruses, despite its known activity against other viral types.
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- Type I interferon (IFN)-induced antiviral Mx proteins typically target single-stranded negative-sense RNA viruses.
- Human MxA protein inhibits measles, human, and bovine parainfluenza-3 viruses (BoPi3V), all in the Paramyxoviridae family.
- Bovine Mx1 protein (BoMx1) shows antiviral activity against vesicular stomatitis virus (VSV).
Purpose of the Study:
- To investigate if the bovine Mx1 protein (BoMx1) pathway is involved in antiviral responses against Paramyxoviridae.
- To determine if the antiviral spectrum of BoMx1 extends to the Paramyxoviridae family.
Main Methods:
- A transgenic Vero cell line with doxycycline-inducible BoMx1 expression was used.
- Cells were infected with various Paramyxoviridae viruses at different multiplicities of infection (moi).
- Cell viability, cytopathic effects (CPEs), and infectious virus yields were assessed.
Main Results:
- Cells expressing BoMx1 showed no significant difference in viability, CPEs, or infectious virus yields compared to non-expressing cells.
- This lack of difference was observed across all tested Paramyxoviridae viruses and moi.
- BoMx1 did not demonstrate inhibitory effects on Paramyxoviridae replication.
Conclusions:
- Bovine Mx1 protein (BoMx1) does not interfere with the replication of Paramyxoviridae viruses.
- The antiviral potential of BoMx1 does not extend to this viral family, contrary to initial hypotheses.
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