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Updated: Aug 18, 2026

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System
Published on: December 21, 2019
[Inhibition of binding peptides on replication of duck hepatitis B virus]
Hong-yu Jia1, Zhi Chen, Lin-fu Zhou
1Institute of Infectious Disease, The First Affiliated Hospital, Key Laboratory of Infectious Disease Ministry of Health, College of Medicine, Zhejiang University, Hangzhou 310003, China. jia-hy@tom.com
Objective:
To study the inhibitory effect of binding peptides on duck hepatitis B virus (DHBV) replication in duck hepatocytes.
Methods:
Specific binding peptides to duck hepatitis B virus polymerase (DHBVP) were screened by phage display technology (PDT), then were sequenced and synthesized. Binding peptides were added into primary culture of duck hepatocytes infected with DHBV in vitro. DHBV-DNA in the cytoplasm, cell nucleus and medium supernatant was assayed over time.
Results:
Seven binding peptides were obtained after 3-round screening by PDT. Duck primary hepatocytes infected by DHBV were treated with above obtained binding peptides. The DHBV-DNA levels in medium supernatant and cytoplasm of duck hepatocytes treated with synthesized peptides (the 3rd and the 6th peptide) were significantly lower than those of control cells (P<0.05).
Conclusion:
Specific binding peptides to DHBVP could inhibit the replication of DHBV.
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