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Updated: Aug 15, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
[Determination of levodropropizine and its pharmacokinetics in human plasma using LC/MS/MS]
Li-mei Zhao1, Li Zhao, Ya-xin Sun
1Laboratory of Clinical Pharmacology, No. 2 Hospital of China Medical University, Shenyang 110004, China. LMZhao16@hotmail.com
Aim:
To develop a rapid and sensitive LC/MS/MS method for the analysis of levodropropizine in plasma and study the pharmacokinetics of levodropropizine in healthy Chinese volunteers.
Methods:
Levodropropizine and zolmitriptan (internal standard, IS) were extracted from plasma samples and chromatographed on a C18 column and detected using a tandem mass spectrometer with a TurboIon Spray ionization interface. Quantitation was performed using multiple reaction monitoring (MRM) of the transitions of the m/z 237 --> m/z 120 for levodropropizine and m/z 288 --> m/z 58 for the IS.
Results:
The limit of quantification of the method for levodropropizine was 0.25 microg x L(-1). The assay was linear over the concentration range from 0.25 to 500.0 microg x L(-1) and intra- and inter-day precision over this range were < 11.4% with good accuracy.
Conclusion:
The method is shown to be accurate, and suitable for clinical pharmacokinetic study of levodropropizine.
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