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Antiproliferative effect of Angelica archangelica fruits
Steinthor Sigurdsson1, Helga M Ogmundsdottir, Sigmundur Gudbjarnason
1Science Institute, University of Iceland, Vatnsmyrarvegur 16, IS-101 Reykjavik, Iceland. sts@raunvis.hi.is
Abstract:
The aim of this work was to study the antiproliferative effect of a tincture from fruits of Angelica archangelica and the active components using the human pancreas cancer cell line PANC-1 as a model. Significant dose-dependent antiproliferative activity was observed in the tincture with an EC50 value of 28.6 microg/ml. Strong antiproliferative activity resulted from the two most abundant furanocoumarins in the tincture, imperatorin and xanthotoxin. The contribution of terpenes to this activity was insignificant. Imperatorin and xanthotoxin proved to be highly antiproliferative, with EC50 values of 2.7 microg/ml and 3.7 microg/ml, respectively, equivalent to 10 and 17 microM. The results indicate that furanocoumarins account for most of the antiproliferative activity of the tincture.
Insights
Angelica archangelica tincture shows significant antiproliferative effects against human pancreatic cancer cells. Its activity is primarily driven by furanocoumarins, imperatorin and xanthotoxin.
Area of Science:
- Phytochemistry
- Cancer Biology
- Pharmacology
Background:
- Pancreatic cancer remains a significant health challenge with limited treatment options.
- Natural products are a promising source for novel anticancer agents.
- Angelica archangelica is traditionally used in herbal medicine.
Purpose of the Study:
- To investigate the antiproliferative effects of Angelica archangelica fruit tincture on human pancreatic cancer cells (PANC-1).
- To identify the active components responsible for the observed anticancer activity.
Main Methods:
- In vitro study using the PANC-1 human pancreatic cancer cell line.
- Treatment with Angelica archangelica tincture and isolated compounds.
- Dose-dependent antiproliferative activity assessment using EC50 values.
Main Results:
- The Angelica archangelica tincture exhibited significant dose-dependent antiproliferative activity with an EC50 of 28.6 microg/ml.
- Imperatorin and xanthotoxin, the main furanocoumarins, were identified as potent antiproliferative agents (EC50: 2.7 and 3.7 microg/ml, respectively).
- Terpenes showed negligible contribution to the antiproliferative effect.
Conclusions:
- Furanocoumarins, specifically imperatorin and xanthotoxin, are the primary drivers of the antiproliferative activity of Angelica archangelica tincture against pancreatic cancer cells.
- These findings support the potential of Angelica archangelica as a source for developing new pancreatic cancer therapies.