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Involvement of microbodies in penicillin biosynthesis
W H Müller1, R A Bovenberg, M H Groothuis
1Institute of Biomembranes, State University of Utrecht, Netherlands.
Biochimica Et Biophysica Acta
|April 22, 1992
Abstract:
Penicillium chrysogenum strains were constructed which express a mutant acyltransferase lacking the putative targeting signal for microbody proteins. The mutated enzyme was located in vacuoles and in neighbouring cytoplasm. Although acyltransferase was expressed in vivo and was active in vitro, the mutants did not produce penicillin. The results demonstrate the involvement of microbodies in penicillin production.