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Relationship between plasma leptin, insulin and tumor necrosis factor alpha in obese children
Zehra Aycan1, Merih Berberoğlu, Gönül Ocal
1Department of Pediatric Endocrinology, Ankara University School of Medicine, Ankara, Turkey. zehraaycan67@hotmail.com
Insights
In obese children, plasma leptin and TNFalpha levels were elevated but not correlated with each other. These markers were also similar in hyperinsulinemic and normoinsulinemic obese groups.
Area of Science:
- Pediatric Endocrinology
- Metabolic Syndrome Research
- Obesity and Inflammation Studies
Background:
- Obesity is linked to metabolic dysfunction and inflammation.
- Leptin and TNFalpha are key adipokines involved in metabolic regulation.
- Understanding their interplay in obese children is crucial.
Purpose of the Study:
- To examine the relationship between plasma leptin and TNFalpha in obese children.
- To compare these markers between hyperinsulinemic and normoinsulinemic obese groups.
- To investigate leptin-insulin correlations and TNFalpha G308A mutation.
Main Methods:
- Evaluated BMI, glucose, insulin, HOMA-IR, leptin, and TNFalpha in obese children and lean controls.
- Compared prepubertal vs. pubertal and male vs. female obese groups.
- Assessed correlations between leptin, BMI, insulin, and TNFalpha.
Main Results:
- Obese children had higher fasting insulin, HOMA-IR, leptin, and TNFalpha than controls.
- Obese females exhibited higher leptin and insulin resistance than males.
- Plasma leptin and TNFalpha were similar in hyperinsulinemic vs. normoinsulinemic obese children.
Conclusions:
- Plasma leptin concentrations did not correlate with TNFalpha levels in obese children.
- Leptin and TNFalpha levels were comparable between hyperinsulinemic and normoinsulinemic obese children.
- Findings suggest distinct roles or regulation pathways in pediatric obesity.
Objectives:
1. To evaluate the relationship between plasma leptin and TNFalpha concentrations in obese children and to assess the differences between hyperinsulinemic and normoinsulinemic groups. 2. To evaluate the relationship between plasma leptin and insulin levels in obese children. 3. To investigate the TNFalpha G308A mutation in obese children.
Methods:
Body mass index (BMI), fasting plasma glucose and insulin levels, oral glucose tolerance test results, homeostasis model assessment of insulin resistance (HOMA-IR) results, and plasma leptin and TNFalpha concentrations were evaluated in obese children (n = 45) and age- and gender-matched, lean healthy controls (n = 40).
Results:
In obese children the fasting insulin, HOMA-IR results, plasma leptin and TNFalpha concentrations were significantly higher than in controls (p <0.05). Furthermore, obese females showed higher plasma leptin and insulin resistance compared to obese males. While plasma leptin, TNFalpha levels and HOMA-IR results were similar in the prepubertal and pubertal groups, insulin levels were significantly higher in the pubertal group. Plasma leptin and TNFalpha concentrations were similar in hyperinsulinemic and normoinsulinemic obese children. In control children, plasma leptin concentrations showed a positive correlation with BMI, age, fasting insulin and HOMA-IR results. In obese children, plasma leptin levels did not correlate with BMI, fasting insulin or TNFalpha.
Conclusion:
Plasma leptin concentrations did not show any correlation with TNFalpha levels in obese children. Furthermore, plasma leptin and TNFalpha concentrations were similar in hyperinsulinemic and normoinsulinemic obese children.
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