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Human granulocyte elastase analysis on a clinical analyser (Greiner G-400) and comparison with the manual
1Ludwig Boltzmann Institut für Pädiatrische Endokrinologie und Immunologie, Wien.
European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
|February 1, 1992
Abstract:
The performance of the human granulocyte elastase (EC 3.4.21.37) determination on the clinical analyser Greiner G-400 was evaluated. The detection limit of the assay is 4.68 micrograms/l. With pooled plasma samples, within-run variation (CV) ranged from 1.3 to 6.0%, between-run imprecision was 2.7 to 6.3%. Comparison with the homogenous immunoactivation manual assay using samples from 40 patients resulted in a very good correlation (r = 0.987). This suggests that the adapted instrumental method is able to replace the manual method for routine analyses.