Fibrinogen adsorption on Pyrex glass tubes: a continuous kinetic study

We present an experimental system to record continuously the adsorption kinetics of radiolabeled proteins, following an earlier study (Voegel et al., Colloids Surfaces 10 (1984) 9). We found results in accordance with the Lévêque equation at a wall shear rate of 50 s-1, for adsorption from fibrinogen solutions in Tyrode's buffer on Pyrex glass tubes. Dependence on concentration in the range 4 to 200 micrograms/mL and on distance to the tube entrance were examined. At the highest concentrations, a second slower regime appeared when coverage exceeded about 0.14 micrograms/cm2.