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Photochemically induced, graded cerebral infarction in the mouse by laser irradiation evolution of brain edema
M Boquillon1, J P Boquillon, J Bralet
1Laboratoire de Pharmacodynamie et Physiologie Pharmaceutique, Faculté de Pharmacie, Université de Bourgogne, Dijon, France.
Abstract:
Cerebral infarction was produced in mice by intravenous injection of a photosensitive dye, rose bengal (10 mg/kg), and by focal illumination of the intact skull surface for 3 min with a laser source, operating at 570 nm with power levels of 2, 5, 10, and 20 mW. Location of infarction was made using 2,3,5-triphenyltetrazolium chloride and the time course of edema in the irradiated cerebral hemisphere was evaluated by measuring water, sodium, and potassium content 4, 24, and 72 hr after irradiation. With power levels of 2 and 5 mW, the infarct was restricted to the cortex, whereas with power levels of 10 and 20 mW, it extended to subcortical regions. Increases in water and sodium and decrease in potassium content were maximal 24 hr after irradiation and depended on the power level. Highly significant correlations were found, 24 hr after irradiation, between the power level and the changes in water and ion concentrations. With reproducible, incremental grades of damage being desirable for pharmacological trials, the model may be suited for the testing of therapeutic agents.
Insights
This study developed a mouse model of cerebral infarction using rose bengal dye and laser irradiation. The laser power level controlled infarct size and edema, showing potential for testing new stroke therapies.
Area of Science:
- Neuroscience
- Biomedical Engineering
- Pharmacology
Background:
- Cerebral infarction, or stroke, is a major cause of disability.
- Developing reliable animal models is crucial for understanding stroke pathophysiology and testing treatments.
- Current models may lack reproducibility or precise control over lesion severity.
Purpose of the Study:
- To establish a reproducible mouse model of cerebral infarction.
- To investigate the dose-dependent effects of laser power on infarct size and cerebral edema.
- To evaluate the utility of this model for pharmacological studies.
Main Methods:
- Cerebral infarction was induced in mice using rose bengal dye and focal laser irradiation (570 nm).
- Varying laser power levels (2-20 mW) were used to create incremental infarcts.
- Infarct location was determined using 2,3,5-triphenyltetrazolium chloride.
- Cerebral edema was assessed by measuring water, sodium, and potassium content at 4, 24, and 72 hours post-irradiation.
Main Results:
- Laser power directly correlated with infarct size, affecting cortical and subcortical regions.
- Cerebral edema, indicated by increased water and sodium and decreased potassium, peaked at 24 hours.
- Edema severity was dependent on laser power, with significant correlations observed.
- Reproducible, graded cerebral damage was achieved.
Conclusions:
- The rose bengal and laser-induced cerebral infarction model offers controllable and reproducible lesion creation.
- This model is suitable for investigating the effects of varying stroke severity on brain tissue.
- The model holds promise for preclinical evaluation of therapeutic agents for stroke.