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Updated: Aug 18, 2026

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
Published on: January 16, 2016
An outbreak of viral meningitis associated with a public swimming pond
A M Hauri1, M Schimmelpfennig, M Walter-Domes
1Government Health Service Institute, Dillenburg, Germany. a.hauri@suah-ldk.hessen.de
Abstract:
From July to October 2001, 215 cases of aseptic meningitis occurred among the inhabitants of the German city of Kassel and neighbouring counties. A matched case-control study identified bathing in a public, nature-like pond during the beginning of the outbreak as a risk factor for disease [matched odds ratio (mOR) 44.8, 95% confidence interval (CI) 3.9-515.6]. Among bathers, patients with meningitis spent more time in the water (mOR 18.8, 95% CI 2.0-174.1) and swallowed water more frequently (mOR = 7.3, 95% CI 0.7-81.8). Of 30 cerebrospinal fluid samples tested, echovirus 30 was cultured from 16, and echovirus 13 from seven. An echovirus 30 sequence obtained from one pond water sample showed a 99% nucleotide and 100% amino-acid homology with patient isolates. This outbreak demonstrates the potential of nature-like swimming ponds to cause widespread community infection with substantial public health impact.
Insights
Bathing in a public, nature-like pond caused an aseptic meningitis outbreak in Germany. Echovirus 30 was identified as the causative agent, highlighting risks associated with recreational water exposure.
Area of Science:
- Public Health
- Infectious Diseases
- Environmental Health
Background:
- Aseptic meningitis outbreaks can pose significant public health challenges.
- Recreational water activities are potential sources of infectious disease transmission.
Purpose of the Study:
- To investigate the cause and risk factors of an aseptic meningitis outbreak in Kassel, Germany.
- To identify the etiological agent responsible for the outbreak.
Main Methods:
- A matched case-control study was conducted.
- Epidemiological data on patient exposures were collected.
- Cerebrospinal fluid and pond water samples were analyzed for viral pathogens.
Main Results:
- 215 cases of aseptic meningitis were reported between July and October 2001.
- Bathing in a public, nature-like pond was identified as a significant risk factor (mOR 44.8).
- Echovirus 30 was isolated from patients and pond water, confirming its role in the outbreak.
Conclusions:
- Nature-like swimming ponds can be a source of widespread community infections.
- This outbreak underscores the importance of monitoring recreational water quality to prevent infectious disease.
- Public health interventions are crucial to mitigate risks associated with environmental exposures.
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