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Macaque trophoblast migration is regulated by RANTES.

Twanda L Thirkill1, Kimberly Lowe, Hemamalini Vedagiri

  • 1Department of Cell Biology and Human Anatomy, School of Medicine, Tupper Hall, One Shields Ave University of California, Davis, CA 95616-8643, USA.

Experimental Cell Research
|April 9, 2005
PubMed
Summary

Regulated on Activation T-Cell Expressed and Secreted (RANTES) and its receptor CCR5 may regulate trophoblast cell migration during early pregnancy. This chemokine signaling pathway influences key cell adhesion molecules, impacting placental development.

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Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Immunology

Background:

  • Trophoblast cells migrate extensively during early pregnancy, invading the uterine lining and vasculature.
  • The precise molecular mechanisms governing this directional trophoblast migration remain largely unknown.
  • Understanding trophoblast migration is crucial for comprehending placental development and pregnancy success.

Purpose of the Study:

  • To investigate the role of the chemokine Regulated on Activation T-Cell Expressed and Secreted (RANTES) in regulating trophoblast cell migration.
  • To determine if the RANTES receptor, CCR5, is expressed by trophoblasts and involved in their migration.
  • To explore the downstream signaling pathways affected by RANTES in trophoblast cells.

Main Methods:

  • Immunohistochemistry and immunocytochemistry to detect RANTES and CCR5 expression in endometrial tissue and isolated cells.

Related Experiment Videos

  • Reverse transcription-polymerase chain reaction (RT-PCR) to confirm gene expression of RANTES and CCR5.
  • In vitro migration assays using trophoblast cells cultured with RANTES and blocking antibodies against CCR5.
  • Analysis of beta1 integrin expression in response to RANTES stimulation.

    • Main Results:

    • RANTES was expressed by stromal and vascular cells in the endometrium, and by isolated endothelial cells.
    • The RANTES receptor, CCR5, was expressed by trophoblasts and other cells within the developing placenta.
    • Trophoblast cells demonstrated directed migration towards RANTES in vitro, which was inhibited by anti-CCR5 antibodies.
    • RANTES stimulation increased beta1 integrin expression in trophoblasts, an effect dependent on pertussis toxin-sensitive signaling.

    Conclusions:

    • RANTES and its receptor CCR5 play a significant role in regulating trophoblast cell migration within the maternal endometrium.
    • This chemokine signaling pathway likely contributes to the invasion of uterine vasculature by trophoblasts.
    • The findings provide novel insights into the molecular mechanisms governing early placental development and vascular integration.