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Related Experiment Videos

Antiphospholipid antibody tests: spreading the net.

M L Bertolaccini1, S Gomez, J F P Pareja

  • 1Lupus Research Unit, The Rayne Institute, St Thomas' Hospital, London SE1 7EH, UK. maria.bertolaccini@kcl.ac.uk

Annals of the Rheumatic Diseases
|April 9, 2005
PubMed
Summary

Testing for antiprothrombin antibodies (aPT) may help diagnose antiphospholipid syndrome (APS) in systemic lupus erythematosus (SLE) patients with thrombosis who are negative for anticardiolipin antibodies (aCL) and lupus anticoagulant (LA). Anti-beta(2)-glycoprotein I (anti-beta(2)GPI) were not found in aCL-negative patients.

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Area of Science:

  • Rheumatology
  • Immunology
  • Hematology

Background:

  • Antiphospholipid syndrome (APS) is a condition characterized by recurrent thrombosis and pregnancy morbidity.
  • Systemic lupus erythematosus (SLE) patients with thrombosis can be challenging to diagnose with APS, especially when traditional antiphospholipid antibody tests are negative.
  • Identifying novel antibody specificities is crucial for improving APS diagnosis in SLE patients.

Purpose of the Study:

  • To investigate whether testing for new antiphospholipid antibody specificities can aid in identifying APS in SLE patients with thrombosis who are persistently negative for anticardiolipin antibodies (aCL) and/or lupus anticoagulant (LA).

Main Methods:

  • Three groups of SLE patients were studied: SLE/APS (n=56), SLE with thrombosis but negative for aCL/LA (n=56), and SLE only (n=56).

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  • Patients were tested for anti-beta(2)-glycoprotein I (anti-beta(2)GPI) and antiprothrombin antibodies (aPT) using specific assays.
  • Conventional aCL and LA tests were re-evaluated in all samples.

    • Main Results:

    • Anti-beta(2)GPI antibodies were exclusively found in SLE/APS patients, all of whom were also aCL positive.
    • Antiprothrombin antibodies (aPT) and antibodies against phosphatidylserine-prothrombin complex (aPS-PT) were significantly more prevalent in the SLE/APS group compared to the other two groups.
    • No significant differences in anti-beta(2)GPI, aPT, or aPS-PT were observed between the SLE/thrombosis and SLE only groups.

    Conclusions:

    • Testing for antiprothrombin antibodies (aPT) may be beneficial for diagnosing APS in selected SLE patients with thrombosis who are persistently negative for aCL and LA.
    • Anti-beta(2)GPI antibodies are not present in SLE patients who are negative for aCL, suggesting they are not a marker for APS in this subgroup.