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c-src activating mutation analysis in Chinese patients with colorectal cancer
Ye-Xiong Tan1, Han-Tao Wang, Peng Zhang
1International Co-operational Laboratory on Signal Transduction, Eastern Hepatobiliary Institute, Shanghai 200438, China.
Aim:
To investigate the occurrence of cellular src (c-src) activating mutation at codon 531 in colorectal cancer patients from Chinese mainland.
Methods:
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay followed by sequencing and single-strand conformation polymor-phism analysis were carried out to screen 110 samples of primary colorectal cancer and 20 colorectal liver metastases.
Results:
Only one sample showed PCR-RFLP-positive results and carried somatic codon 531 mutations. No additional mutation of c-src exon 12 was found.
Conclusion:
c-src codon 531 mutation in colorectal cancer is not the cause of c-src activation.
