[A fluorometric method for the determination of norepinephrine in tissue]

Area of Science:

• Biochemistry
• Analytical Chemistry
• Neuroscience

Background:

• Norepinephrine (NE) is a critical neurotransmitter and hormone.
• Accurate quantification of NE in biological tissues is essential for research.
• Existing methods may lack sensitivity or simplicity.

Purpose of the Study:

• To develop and validate a spectrofluorometric method for norepinephrine determination in tissue.
• To establish the sensitivity, linearity, and recovery rate of the proposed method.

Main Methods:

• Spectrofluorometry was employed for NE quantification.
• Excitation and emission wavelengths were optimized (lambdaex/lambdaem = 410 nm/505 nm).
• Linearity was assessed in the range of 10-100 ng of NE.

Main Results:

• A linear correlation was observed between fluorescence intensity and NE content.
• Recovery rates ranged from 91.68% to 96.64%.
• Relative Standard Deviation (RSD) was 6.1% (n=10), indicating good precision.

Conclusions:

• The developed spectrofluorometric method is simple, rapid, and sensitive for NE determination.
• The method provides accurate and reproducible quantification of norepinephrine in tissue samples.
• This technique is suitable for routine biochemical analysis and research applications.