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[The investigation for lysozyme conformation changes in deuteromethanol solution by FT-Raman spectrometer]
1Institute of Chemical Metallurgy, Chinese Academy of Sciences, 100080 Beijing.
Abstract:
Lysozyme conformation in deuteromethanol-deuteroxide solution has been studied by FT-Raman spectrometer. By using amide I mode in the Raman spectra and curve fitting method, information of Lysozyme's secondary structure is easily detected in details, and tertiary structure changes can be suggested by relative intensity changes of some bands. It is concluded that lysozyme conformation may be changed to loose structure with incrasing deuteromethanol concentration, and it seems like that increasing loose beta-sheet content lead to the gel's appearing in the solution.
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