[Study on the ternary complex of CPFX, copper (II) and DNA by the method of fluorescence]

Using the method of fluorescence spectrometry, the formation of binary complexes of CPFX-Cu(II) and CPFX-eucaryote DNA, and ternary complexes of CPFX-Cu(II)-eucaryote DNA was studied. The results show that the fluorescent intensity of CPFX can be quenched in the presence of Cu(II ) or DNA in CPFX system. In the presence of Cu (II), the fuorescence quenching action of DNA on CPFX was enhanced strongly. Based on the fluorescent intensity, the formation constants of CPFX-Cu (II) and CPFX-DNA in either the presence or absence were calculated,and the mechanism of interaction among CPFX,Cu(II) and DNA was presented.