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Related Concept Videos

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and refractory oxide ion...
Gas Chromatography: Sample Injection Systems01:08

Gas Chromatography: Sample Injection Systems

In gas chromatography, the sample is introduced as a vapor plug into the carrier gas stream for high efficiency and resolution. A microsyringe injects the sample solution into a heated sample port, vaporizing it and mixing it with the carrier gas. This process is important to ensure the sample is properly prepared for analysis. Thermally sensitive samples can be injected directly into the column and volatilized by slowly increasing the column temperature.
Two primary injection methods are used...
Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...

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Related Experiment Video

Updated: Jul 11, 2026

Design and Construction of an Urban Runoff Research Facility
13:48

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Published on: August 8, 2014

[Flow injection analysis for trace phosphorus(V) and arsenic(V) determination].

G Song1

  • 1Analysis Center, Hubei University, 430062 Wuhan.

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|April 12, 2005
PubMed
Summary

A new flow injection analysis method detects trace phosphorus (P(V)) and arsenic (As(V)) using rhodamine 6G fluorescence quenching. This technique accurately quantifies these elements in copper alloys.

Area of Science:

  • Analytical Chemistry
  • Spectroscopy

Context:

  • Accurate determination of trace phosphorus (P(V)) and arsenic (As(V)) is crucial in various industrial and environmental applications.
  • Existing analytical methods may face limitations in sensitivity or sample throughput for these specific analytes.

Purpose:

  • To establish a sensitive and reliable flow injection analysis (FIA) method for the simultaneous determination of trace P(V) and As(V).
  • To utilize the fluorescence quenching effect of rhodamine 6G for sensitive detection.

Summary:

  • A novel FIA method was developed based on the fluorescence quenching of rhodamine 6G by ion-association complex formation with P(V) and As(V).
  • The method exhibits linear calibration ranges of 0-80 µg/L for P(V) and 0-100 µg/L for As(V).
  • Rhodamine 6G shows maximum emission at 555 nm upon excitation at 350 nm.

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Impact:

  • The developed method provides a robust analytical tool for quantifying trace P(V) and As(V) in complex matrices like copper alloys.
  • Demonstrates the utility of rhodamine 6G-based fluorescence quenching in trace element analysis.
  • Offers satisfactory results for P(V) and As(V) determination in copper alloys, aiding quality control and material analysis.