[Determination and analysis of physical factors affecting insulin fluorescent spectrum]

Insulin is an important factor which regulates cell metabolism and growth. The influences on insulin fluorescent spectrum by pulse electrical field, concentration and temperature were determined in this paper. It was proved that pulse electrical field, concentration and temperature could influence insulin fluorescent spectrum, and thus influence insulin confirmation and insulin biological activity. The mechanism of insulin influenced by pulse electrical field is different to temperature. It is possible that the electrical field influences the insulin fluorescent spectrum by changing Tyr position and the temperature influences the insulin fluorescent spectrum by molecule thermal motion. The insulin confirmation influenced by electrical field is steady.