Related Experiment Video
Updated: Aug 18, 2026
Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
Published on: November 8, 2016
Production of monoclonal antibodies for detection of Vibrio harveyi
Wannipa Phianphak1, Sirirat Rengpipat, Sombat Rukpratanporn
1Biotechnology Program, National Center for Genetic Engineering and Biotechnology, Ministry of Science and Technology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Abstract:
Monoclonal antibodies (MAbs) against Vibrio harveyi were produced from mice immunized with heat-killed and SDS-mercaptoethanol-treated highly virulent V. harveyi 639. Fifteen MAbs were selected and sorted into 6 groups according to their specificity to various proteins of apparent molecular weight ranging from 8 to 49 kDa. Some antibodies were used for detection of V. harveyi at concentrations as low as 10(4) CFU ml(-1) using immunodot blots. Most of the selected MAbs did not show cross-reactivity to other Vibrio species and other gram-negative bacteria tested. Only 1 MAb (VH39-4E) showed slight cross-reactivity to Aeromonas hydrophila. Another MAb (VH24-8H) bound lightly to V. harveyi 1526 but strongly to V. harveyi 639, allowing rapid differentiation. Two of the MAb groups were used to localize V. harveyi in tissues of infected black tiger shrimp Penaeus monodon by immunohistochemistry. This study demonstrates the versatility of a highly specific immunological tool for the detection of V. harveyi in aquaculture and opens the way for further development of convenient test kits.
Insights
Researchers developed specific monoclonal antibodies (MAbs) to detect Vibrio harveyi, a pathogen affecting aquaculture. These MAbs enable sensitive detection and differentiation of V. harveyi in shrimp, aiding disease management.
Area of Science:
- Immunology
- Microbiology
- Aquaculture Science
Background:
- Vibrio harveyi is a significant pathogen in aquaculture, causing substantial economic losses.
- Accurate and rapid detection methods for V. harveyi are crucial for disease management in shrimp farming.
Purpose of the Study:
- To produce and characterize monoclonal antibodies (MAbs) specific to Vibrio harveyi.
- To evaluate the utility of these MAbs for detecting and differentiating V. harveyi in various applications.
Main Methods:
- Production of MAbs in mice immunized with V. harveyi.
- Specificity testing against various Vibrio species and other bacteria.
- Detection of V. harveyi using immunodot blots and immunohistochemistry.
Main Results:
- Fifteen MAbs were generated, showing specificity to different V. harveyi proteins.
- MAbs detected V. harveyi at concentrations as low as 10^4 CFU/ml.
- High specificity was observed, with minimal cross-reactivity to other bacterial species.
- One MAb demonstrated rapid differentiation between V. harveyi strains.
- MAbs were successfully used for immunohistochemical localization of V. harveyi in shrimp tissues.
Conclusions:
- Monoclonal antibodies provide a versatile and specific tool for detecting Vibrio harveyi.
- These MAbs have potential applications in aquaculture disease diagnostics and management.
- Further development of MAb-based test kits is warranted for convenient V. harveyi detection.
