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Published on: June 15, 2013
IL-1beta stimulates argininosuccinate synthetase gene expression through NF-kappaB in Caco-2 cells
Carole Brasse-Lagnel1, Alain Lavoinne, Alain Fairand
1Groupe Appareil Digestif, Environnement et Nutrition, EA 3234, Institut Fédératif de Recherches Multidisciplinaires sur les Peptides (23), Université de Rouen, Rouen, France.
Abstract:
Argininosuccinate synthetase (ASS) is limiting the arginine synthesis and can be stimulated by immunostimulants. We previously identified a putative NF-kappaB element in the human ASS gene promoter but its functionality was unknown (Husson et al., Eur. J. Biochem. 270 (2003) 1887). In the present study, using Caco-2 cells, a human enterocyte line, we demonstrate that IL-1beta rapidly induces the expression of the ASS gene at a transcriptional level through NF-kappaB activation. Using gel shift assay and double-strand oligonucleotide sequence of the identified putative NF-kappaB binding site of the ASS promoter, we provide evidence that NF-kappaB may functionally interact with this element.
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