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[Epidemic keratoconjunctivitis. Detecting adenoviruses].
J Mielke1, M Grüb, N Freudenthaler
1Abteilung 2, Sektion für Motilitätsstörungen, Universitäts-Augenklinik Tübingen. joerg.mielke@med.uni-tuebingen.de
Summary
The rapid SAS Adenotest accurately detects adenovirus in epidemic keratoconjunctivitis, showing 72% sensitivity and 100% specificity. Further polymerase chain reaction (PCR) testing is recommended for persistent symptoms.
Area of Science:
- Ophthalmology
- Virology
- Diagnostic Assays
Background:
- Epidemic keratoconjunctivitis (EKC) is a common viral eye infection.
- Adenovirus is the primary cause of EKC.
- Rapid and accurate diagnosis is crucial for patient management and infection control.
Purpose of the Study:
- To evaluate the diagnostic accuracy of the SAS Adenotest, a rapid 15-minute adenovirus assay.
- To compare the SAS Adenotest's performance against polymerase chain reaction (PCR) for adenovirus detection.
Main Methods:
- Conjunctival swabs were collected from 75 patients with suspected EKC.
- Adenovirus detection was performed using both the SAS Adenotest and PCR.
Main Results:
- Adenovirus was detected by PCR in 25 patients.
- The SAS Adenotest correctly identified 18 of these cases (72% sensitivity).
- The assay demonstrated 100% specificity with no false positive results.
Conclusions:
- The SAS Adenotest is a valuable tool for the rapid diagnosis of adenovirus in early EKC.
- Persistent symptoms beyond 5 days warrant confirmatory PCR testing.
- The assay aids in timely clinical decisions and infection control.