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[Study on the ingredients of reserpine by TLC-FT-SERS]

Y Wang1, F Zi, Y Wang

  • 1Chemistry Department, Capital Normol University, 100037 Beijing.

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|April 13, 2005
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Thin-Layer Chromatography (TLC): Overview

Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...