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Related Experiment Videos

Robust quantification of in vitro angiogenesis through image analysis.

Antti Niemistö1, Valerie Dunmire, Olli Yli-Harja

  • 1antti.neimisto@tut.fi

IEEE Transactions on Medical Imaging
|April 13, 2005
PubMed
Summary

An automated image analysis method quantifies in vitro angiogenesis, crucial for cancer drug discovery. This robust software accurately measures tubule formation and junctions, aiding anti-angiogenic agent research.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Cancer Research

Background:

  • In vitro angiogenesis assays are vital for identifying anti-angiogenic cancer therapies.
  • Accurate quantification of endothelial cell and fibroblast co-cultures is essential for assessing drug efficacy.
  • Existing methods may lack precision in measuring complex tubule structures.

Purpose of the Study:

  • To present an automated image analysis method for quantifying in vitro angiogenesis.
  • To provide accurate measurements of tubule complex lengths, sizes, and junction numbers.
  • To develop a robust software tool for analyzing angiogenesis assays.

Main Methods:

  • Development of an automated image analysis algorithm for in vitro angiogenesis.
  • Application of the method to co-cultures of endothelial cells and fibroblasts.

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  • Testing with a commercially available angiogenesis assay and validation with known agents.

    • Main Results:

    • The method accurately quantifies tubule complex morphology and junction formation.
    • Demonstrated inhibitory effect of suramin and stimulatory effect of vascular endothelial growth factor.
    • Image analysis proved robust against variations in illumination.

    Conclusions:

    • The automated method provides accurate and robust quantification of in vitro angiogenesis.
    • The developed software facilitates research in anti-angiogenic drug discovery.
    • The software and test images are publicly available for research use.