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Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
Published on: December 20, 2010
SCAPE: a new tool for the Selective CApture of PEptides in protein identification
Lazaro Betancourt1, Jeovanis Gil, Vladimir Besada
1Department for Proteome Analysis, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana, Cuba. lazaro.betancourt@cigb.edu.cu
Abstract:
A method for the selective capture and analysis of peptides containing neither histidine nor arginine is evaluated. It is based on the reversible modification of alpha- and epsilon-amino groups of peptides and the relatively easy separation of charged and noncharged peptides by cation exchange chromatography. The simplicity of the method and the results obtained in silico and with standard proteins, anticipate the utility of the SCAPE approach for proteome analyses.

