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Physicochemical characterization of vibriophage N5
1Division of Electron Microscopy, National Institute of Cholera and Enteric Diseaess, Beleghata, Kolkata- 700010, India. sena@mail.nih.gov
Virology Journal
|April 13, 2005
Abstract:
Phage N5 is one of the phages of Vibrio cholerae serovar O1 biotype El Tor (Ghosh, A. N., Ansari, M. Q., and Dutta, G. C. Isolation and morphological characterization of El Tor cholera phages. J. Gen. Virol. 70: 2241-2243, 1989). In the present communication the growth curve, molecular weight and confirmation of the genome, partial denaturation map and restriction endonuclease digestion pattern have been determined. Partial denaturation map indicates that the genome has non-permuted / invariant sequence. Presence of cohesive ends has also been documented.