Bioactivity quantification of crude bacteriocin solutions
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Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials
Published on: April 10, 2016
Amélia Delgado1, Dulce Brito, Pedro Fevereiro
1Instituto de Tecnologia Química e Biológica, Department de Microbiology, Av. da República, Quinta do Marquês 2780-505 Oeiras, Portugal. ameliad@itqb.unl.pt
Journal of Microbiological Methods
|April 13, 2005
Abstract:
Bacteriocin activity can be quantified by a simple parallel line model based on an agar diffusion assay. The quotient between the inhibition zone area and the sensitivity of the indicator bacterium can be used to compare different bacteriocins. Deferred calculations of relative potencies are possible, once purified compounds are obtained.
