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Published on: October 8, 2013
Rapid protocol for electroporation of Clostridium perfringens
Alena Jirásková1, Libor Vítek, Johan Fevery
1Institute of Microbiology AS CR, Vídenská 1083, 14220 Prague 4, Czech Republic.
Journal of Microbiological Methods
|April 13, 2005
Abstract:
A rapid and simple method has been developed for the electroporation of Clostridium perfringens with plasmid DNA. The new improvements, harvesting cells early in the logarithmic stage of growth, keeping the cells at room temperature and the absence of post-shock incubation on ice increased transformation efficiency by one order of magnitude.
