Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Experimental research on laser-induced single-molecule detection].

L Zhang1, G Liu, W Ma

  • 1Center of Atomic and Molecular Sciences, Department of Physics, Tsinghua University, 100084 Beijing.

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|April 14, 2005
PubMed
Summary

This study introduces a laser-induced single-molecule detection instrument for ultra-sensitive analysis. Experiments with C445 dye demonstrate its capabilities and limitations, including photon bleach effects, paving the way for future research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oscillation and light induction of timeless mRNA in the mammalian circadian clock.

The Journal of neuroscience : the official journal of the Society for Neuroscience·1999
Same author

Distributions of nicotinic acetylcholine receptor alpha7 and beta2 subunits on cultured hippocampal neurons.

Neuroscience·1999
Same author

In vitro effects of zinc on markers of bone formation.

Biological trace element research·1999
Same author

Intracameral muramyl dipeptide-induced paracellular permeability associated with decreased glutamate transporter and gamma -glutamyltranspeptidase activities.

Experimental eye research·1999
Same author

Specific regulation of fos family transcription factors in thymocytes at two developmental checkpoints.

International immunology·1999
Same author

A study on continuous low-flow perfusion with low-potassium dextran for donor isolated lung preservation.

Chinese medical journal·1999

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Biophysics

Context:

  • Single-molecule detection offers ultra-high sensitivity for various applications.
  • Existing techniques face challenges in sensitivity and specific applications.
  • Advancements in instrumentation are crucial for pushing detection limits.

Purpose:

  • To report the principle and device of a novel laser-induced single-molecule detection spectrum instrument.
  • To present experimental results validating the instrument's performance.
  • To analyze factors affecting detection and discuss limitations like photon bleach.

Summary:

  • A laser-induced single-molecule detection spectrum instrument was developed.
  • Experiments using C445 dye evaluated sample velocity, energy characteristics, linearity, and detection limits.

Related Experiment Videos

  • The impact of photon bleach on experimental outcomes was investigated.

    • Impact:

    • Provides a foundation for future research in ultra-sensitive detection.
    • Enables advancements in fields requiring single-molecule analysis, such as molecular diagnostics and environmental monitoring.
    • Highlights the importance of addressing photon bleach for accurate single-molecule measurements.