Related Experiment Video
Updated: Jun 24, 2026

A Colorimetric Method for Measuring Iron Content in Plants
Published on: September 7, 2018
[Study on colour reaction of iron(II)-thibarbituric acid-nitrite system and its analytical application]
1Health and Antiepid Station, Xingshan County, 443700 Xingshan.
Abstract:
In this paper, the optimum conditions of colour reaction of iron (II)-thibarbituric acid-NO2(-) system, existent state and spectrophotometric characteristies of the complex were studied, and the reaction mechanism discussed. A new spectrophotometric method for the determination of trace iron was established. In a buffer solution of borate/NaOH of pH9.5, iron (II) and thibarbituric acid-NO2(-) react to form a stable blue complex anion, for which the maximum absorbance is at 645nm and the molar absorptivity is 2.66 x 10(4)L x mol(-1) x cm(-1). Beer's law is obeyed in the range of 0-40 microg/25mL for iron (II). The method is simple and rapid, shows satisfactory selectivity and precision and has been applied to determine iron in waters with satisfactory results.
More Related Videos
06:19Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
09:51TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples
Published on: September 19, 2025
Related Concept Videos
Classification of Titrimetric Analysis Based on Reaction Types
Titrations between an acid and a base lead to neutralization reactions that form...
Redox Titration: Overview
Redox Titration: Iodimetry and Iodometry
Redox Titration: Other Oxidizing and Reducing Agents
Complexometric Titration: Overview
Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...