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Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
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[Study on the interaction of lauryl sodium sulfate and rhodamine-6G before micellar formation]
Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|April 14, 2005
Abstract:
The absorption spectra of an aqueous solution of Lauryl sodium sulfate (SDS) and Rhodamine-6G (R6G) over a wide concentration range were studied. The results show that when c(sds) < 1 x 10(-4)mol x L(-1) SDS forms complex with R6G with molar ratio of 1 : 1, but when c(sds) > 1 x 10(-4) mol x L(-1) the premicelle, which affects the aggregate state of R6G obviously, is formed in the solution. With SDS present, the aggregation number and aggregation constant of R6G were determined, and the dissociating of R6G by inorganic salts also investigated.
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